Incubator vs. Autoclave — What's the Difference?
By Tayyaba Rehman — Updated on December 29, 2023
An incubator is a device used to grow and maintain microbiological cultures or cell cultures at a constant temperature, while an autoclave is a device used to sterilize equipment and supplies by subjecting them to high-pressure saturated steam.
Difference Between Incubator and Autoclave
Table of Contents
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Key Differences
An incubator is designed to create optimal environmental conditions for the growth and maintenance of biological cultures. An autoclave is primarily used for sterilization, utilizing high-pressure steam to eliminate bacteria, viruses, and other microorganisms.
Incubators operate at controlled temperatures and sometimes controlled humidity and CO2 levels to support cell growth. Autoclaves use high temperatures (usually above 121°C) and pressure to achieve sterilization.
Incubators are vital in laboratories for culturing cells, bacteria, and embryos, often used in research and medical settings. Autoclaves are essential in hospitals, laboratories, and other settings requiring sterile conditions, used for sterilizing instruments, glassware, and even waste.
An incubator is built to maintain a stable, controlled environment suitable for biological processes. An autoclave is constructed to withstand high pressure and temperatures, featuring sealed chambers and safety valves.
The use of incubators is crucial for growing cultures that can be used for experiments, diagnostics, and treatments. Autoclaves are critical for ensuring the safety of medical procedures and laboratory experiments by providing sterile equipment.
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Comparison Chart
Primary Function
Growing and maintaining cultures
Sterilizing equipment and supplies
Operating Principle
Controlled temperature, humidity, and CO2
High-pressure saturated steam
Typical Use
Culturing cells, bacteria, embryos
Sterilizing surgical instruments, glassware
Design Characteristics
Stability in environmental conditions
Withstands high temperature and pressure
Role in Health and Safety
Culturing for research and diagnostics
Ensuring sterility for safety
Compare with Definitions
Incubator
A device for growing and maintaining microbiological cultures.
The lab used an incubator to grow bacterial cultures.
Autoclave
A device for sterilization using high-pressure steam.
The hospital autoclave sterilized surgical instruments.
Incubator
An apparatus used to maintain environmental conditions for cell growth.
Scientists placed the cell cultures in an incubator.
Autoclave
A high-pressure chamber used for sterilizing laboratory waste.
Biological waste was disposed of safely after autoclaving.
Incubator
Equipment used in neonatal units to maintain optimal conditions for premature babies.
The premature baby was kept in an incubator for warmth and care.
Autoclave
A machine used in laboratories to sterilize equipment.
After use, the lab glasses were placed in the autoclave.
Incubator
A machine that provides controlled temperature and humidity for hatching eggs.
The farmer used an incubator to hatch chicken eggs.
Autoclave
A sterilization tool used in tattoo parlors and dental clinics.
The autoclave ensured that the tattoo needles were free of contaminants.
Incubator
A tool in biotech and medical research for cultivating biological samples.
The research study required the use of an incubator for tissue culture.
Autoclave
Equipment for killing bacteria and viruses on medical tools.
The autoclave is essential for maintaining a sterile environment.
Incubator
An enclosed apparatus in which premature or unusually small babies are placed and which provides a controlled and protective environment for their care.
Autoclave
An autoclave is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure/temperature. Autoclaves are used in medical applications to perform sterilization and in the chemical industry to cure coatings and vulcanize rubber and for hydrothermal synthesis.
Incubator
A place, especially with support staff and equipment, made available at low rent to new small businesses
An incubator project
Autoclave
A strong, pressurized, steam-heated vessel, as for laboratory experiments, sterilization, or cooking.
Incubator
An apparatus in which environmental conditions, such as temperature and humidity, can be controlled, often used for growing bacterial cultures, hatching eggs artificially, or providing suitable conditions for a chemical or biological reaction.
Autoclave
A strong, pressurized, heated vessel, as for laboratory experiments, sterilization, cooking or mineral processing.
Incubator
(Medicine) An apparatus for maintaining an infant, especially a premature infant, in an environment of controlled temperature, humidity, and oxygen concentration.
Autoclave
(transitive) To sterilize laboratory equipment in an autoclave.
Incubator
A place or situation that permits or encourages the formation and development, as of new ideas
A university that was an incubator of new approaches to sociology.
Autoclave
(cryptography) autokey
Incubator
An organization that provides new businesses with technical and support services and usually low-cost office space or infrastructure.
Autoclave
A kind of French stewpan with a steam-tight lid.
Incubator
(chemistry) Any apparatus used to maintain environmental conditions suitable for a reaction.
Autoclave
A device used for sterilizing objects by exposing them to steam at above atmospheric pressure (and thus at a temperature above the normal boiling point of water). It consists of a closed chamber able to withstand internal pressure, and a means of passing superheated steam into the chamber. Autoclaves are made in various sizes, and are much used in hospitals and research laboratories to render instruments and equipment sterile.
Incubator
(medicine) An apparatus used to maintain environmental conditions suitable for a newborn baby.
Autoclave
A device for heating substances above their boiling point; used to manufacture chemicals or to sterilize surgical instruments
Incubator
An apparatus used to maintain environmental conditions suitable for the hatching of eggs.
Autoclave
Subject to the action of an autoclave
Incubator
A place to maintain the culturing of bacteria at a steady temperature.
Incubator
(business) A support programme for the development of entrepreneurial companies.
Incubator
That which incubates, especially, an apparatus by means of which eggs are hatched by artificial heat.
Incubator
An apparatus containing an enclosed chamber, used for the cultivation of microörganisms or tissue cultures by maintaining a suitable temperature and atmospheric composition. Some incubators have no provision for maintaining a special atmosphere, while in others, especially for anaerobic organisms and tissue culture, the moisture level and composition of the gases are also controlled.
Incubator
An apparatus consisting of enclosed chamber, for maintaining prematurely born babies in a favorable environment until able to thrive under normal conditions. The temperature and level of oxygen in the atmosphere may be controlled.
Incubator
Apparatus consisting of a box designed to maintain a constant temperature by the use of a thermostat; used for chicks or premature infants
Common Curiosities
How hot does an autoclave get?
Typically, autoclaves operate at temperatures above 121°C.
Can incubators regulate CO2 levels?
Some advanced incubators can control CO2 levels for specific culture needs.
Do autoclaves use chemicals for sterilization?
No, autoclaves primarily use high-pressure steam for sterilization.
Is an autoclave necessary in every laboratory?
In most labs, especially those dealing with biological materials, autoclaves are essential.
Can an autoclave sterilize any material?
Autoclaves are suitable for heat-resistant materials but not for heat-sensitive items.
Are incubators used in plant research?
Yes, for germinating seeds and growing plant tissues.
Can incubators be used for anything besides cultures?
Yes, they're also used for hatching eggs and caring for premature babies.
Are incubators found in hospitals?
Yes, particularly in neonatal units for premature infants.
Can incubators simulate different environmental conditions?
Advanced models can simulate various conditions, including temperature and humidity.
Do incubators require monitoring?
Yes, regular monitoring is necessary to ensure optimal conditions.
How long does an incubator take to grow cultures?
It varies, depending on the type of culture and conditions set.
Can autoclaves be dangerous?
If not used properly, the high pressure and temperature can be hazardous.
Are autoclaves used in food processing?
Yes, for sterilizing canned food and beverages.
Do all medical clinics have an autoclave?
Most clinics, especially those performing procedures, have an autoclave for sterilization.
How often do autoclaves need maintenance?
Regular maintenance is crucial for safety and efficacy.
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Written by
Tayyaba RehmanTayyaba Rehman is a distinguished writer, currently serving as a primary contributor to askdifference.com. As a researcher in semantics and etymology, Tayyaba's passion for the complexity of languages and their distinctions has found a perfect home on the platform. Tayyaba delves into the intricacies of language, distinguishing between commonly confused words and phrases, thereby providing clarity for readers worldwide.